Association of polymorphisms of NAPE-PLD and FAAH genes with schizophrenia in Chinese Han population.
- Author:
Peiru SI
1
;
Shulian LIU
;
Dongxiao TONG
;
Meijin CHENG
;
Liwen WANG
;
Xiaoli CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Amidohydrolases; genetics; Asian Continental Ancestry Group; genetics; China; ethnology; Female; Genotype; Humans; Male; Middle Aged; Phospholipase D; genetics; Polymorphism, Genetic; Schizophrenia; genetics
- From: Chinese Journal of Medical Genetics 2018;35(2):215-218
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo assess the association of polymorphisms of N-acyl-phosphatidylethanolamine-phospholipase D (DAPE-PLD) and fatty acid amide hydrolase (FAAH) genes, as well as their interaction, with schizophrenia.
METHODSPolymorphisms of NAPE-PLD rs12540583 and FAAH rs324420, rs2295633, and rs6429600 were determined with PCR - restriction fragment length polymorphism assay and Sanger sequencing. The genotypes of 345 subjects of Han Chinese origin diagnosed with schizophrenia and a 403 controls were compared. The results were analyzed with SPSS 17.0, and the interaction of the two genes was analyzed using a multifactor dimensionality reduction (MDR) method.
RESULTSThe frequency of NAPE-PLD rs12540583 polymorphism was significantly different between the two groups under both dominant and additive models (χ2=17.18 vs. χ2=18.94, P<0.0125). The frequencies of AC genotype and C allele of the patient group at rs12540583 were higher than those of the controls, and the interaction of NAPE-PLD and FAAH was associated with schizophrenia. A four-loci model (rs12540583, rs324420, rs2295633 and rs6429600) can best model the interaction between NAPE-PLD and FAAH.
CONCLUSIONThe AC genotype and C allele of NAPE-PLD rs12540583 locus are risk factors for schizophrenia, and the interaction between NAPE-PLD rs12540583 and FAAH rs324420, rs2295633 and rs6429600 is associated with schizophrenia.
