Effects of FBI-1 silencing on proliferation and apoptosis of triple-negative breast cancer cell line MDA-MB-231.
- Author:
Mao-Jian CHEN
1
;
Li WANG
2
;
Wei-Ping YANG
1
;
Qing-Hong QIN
1
;
Qi-Xing TAN
1
;
Bin LIAN
1
;
Chang-Yuan WEI
3
Author Information
1. Department of Breast Surgery, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China.
2. Department of Breast Surgery, Liuzhou People's Hospital, Liuzhou 545006, China.
3. Department of Breast Surgery, The Affiliated Tumor Hospital of Guangxi Medical University, Nanning 530021, China. weicy63@aliyun.com.
- Publication Type:Journal Article
- From:
Acta Physiologica Sinica
2018;70(5):497-503
- CountryChina
- Language:Chinese
-
Abstract:
This work aimed to observe the effects of short hairpin RNA (shRNA)-silenced FBI-1 (factor that binds to the inducer of short transcripts of human immunodeficiency virus-1) on proliferation and apoptosis of triple-negative breast cancer cell line MDA-MB-231. qRT-PCR and Western blot analysis were applied to detect the mRNA and/or protein expression of FBI-1, Bcl-2, Bax, cleaved-Caspase 3 and Survivin. RNA interference method was used to silence FBI-1 expression in MDA-MB-231 cells. CCK-8 and colony formation assay were employed to detect the cell proliferation. Flow cytometry was employed for examining cell apoptosis. In vivo tumorigenicity of MDA-MB-231 cells was detected by tumor transplantation in nude mice. The results showed that the mRNA and protein expressions of FBI-1 were higher in MDA-MB-231 cells compared with those in normal human mammary epithelial cells MCF-10A. FBI-1 gene silencing inhibited proliferation and induced apoptosis of MDA-MB-231 cells in vitro, together with decreased Bcl-2 and Survivin protein expression, increased Bax protein expression and activated Caspase 3. Moreover, FBI-1 gene silencing inhibited the tumorigenesis of MDA-MB-231 cells in vivo. These results suggest that silencing of FBI-1 gene inhibits proliferation, induces apoptosis and suppresses the tumorigenesis of MDA-MB-231 cells.
