Expression, purification, characterization and application of α-amino acid ester acyltransferase from recombinant Escherichia coli.
- Author:
Pengfei LIU
1
;
Qimeng LU
1
;
Xueqin HU
1
;
Xuewen HOU
1
;
Hongbin ZHANG
1
Author Information
- Publication Type:Journal Article
- Keywords: L-alanyl-L-glutamine; catalysis conditions; characterization; expression conditions; α-amino acid ester acyltransferase
- From: Chinese Journal of Biotechnology 2018;34(7):1169-1177
- CountryChina
- Language:Chinese
- Abstract: α-Amino acid ester acyltransferase (Aet) catalyzes the L-alanyl-L-glutamine forming reaction from L-alaine methylester hydrochloride and L-glutamine. In this study, the recombinant Escherichia coli saet-QC01 was used to express the α-amino acid acyltransferase, and its expression conditions were optimized. The recombinant protein was separated and purified by Ni-NTA affinity chromatography, and its enzymatic properties and catalytic applications were studied. The induction conditions suitable for enzyme production optimized were as follows: The temperature was 20 ℃, the induction stage (OD₆₀₀=2.0-2.5), IPTG concentration was 0.6 mmol/L, induction time was 12 h. The optimal reaction conditions of α-amino acid acyltransferase were 27 ℃, pH 8.5, it was most stable between pH 7.0 and 8.0 and relatively stable in an acidic environment, and low concentration of Co²⁺ or EDTA could promote the enzyme activity. Under optimal reaction conditions, 600 mmol/L of L-alaine methylester hydrochloride and 480 mmol/L of L-glutamine, the yield of L-alanyl-L-glutamine reached 78.2 g/L and productivity of 1.955 g/L/min, the conversion rate reached 75.0%. α-Amino acid ester acyltransferase has excellent acid-basei resistance, high catalytic efficiency. These characteristics suggest its application prospects in the industrial production.
