Efficacy enhancement of a Baculovirus-vectored Newcastle Disease Virus F protein vaccine by chicken GM-CSF and IL-2.

Author: Hang YU ¹ ; Dongni GAO ¹ ; Yan SHEN ¹ ; Ying LIU ¹ ; Wenxiang PING ¹ ; Jingping GE ¹
Author Information

1. Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, Heilongjiang, China.
Publication Type:Journal Article
Keywords: GM-CSF; IL-2; Newcastle disease; co-immunization; immune effect
From: Chinese Journal of Biotechnology 2018;34(9):1442-1452
CountryChina
Language:Chinese
Abstract: To compare with the effects of the GM-CSF and IL-2 used as adjuvants in the baculovirus vaccine, we used genetic engineering to construct the recombinant baculovirus rBV-LMI-F and with GM-CSF and IL-2 to immunized chickens. Then, we compared the concentration of the neutralizing antibody and cytokines to determine the immunostimulatory effects of GM-CSF and IL-2. GM-CSF induced higher levels of antibodies and cytokines in chickens at 28 d and 42 d post-vaccination. In conclusion, GM-CSF could elicit higher serum antibody and cytokines responses and improved the effects of Baculovirus vaccine.