Vancomycin-based fluorescent enzyme-linked immunoabsorbent assay for detection of Staphylococcus aureus.
- Author:
Shuang LI
1
;
Chaochao CHEN
1
;
Xuelan CHEN
1
Author Information
- Publication Type:Journal Article
- Keywords: Staphylococcus aureus; fluorescent enmyze-linked immunosorbent assay; pig IgG; quantum dot nanobeads; vancomycin
- From: Chinese Journal of Biotechnology 2018;34(9):1510-1517
- CountryChina
- Language:Chinese
- Abstract: In the study, fluorescent enzyme-linked immnoabsorbent assay for detection of Staphylococcus aureus was established with IgG from pig as capture antibody and quantum dot nanobeads (QBs) labeled vancomycin (QB-Vans) as testing antibody. Quantum dot of about 100 nm partical size nanobeads were prepared and linked with vancomycin. The optimum concentrations of salt ions were 0.01 mol/L, and the optimum pH was 6.0. Under the optimum conditions, the detection sensitivity for S. aureus was 10⁴ CFU/mL, and there was no cross-reaction with other pathogenic bacteria. Thus, the method could be used for rapid screening of S. aureus, for the clinical monitoring and foodborne pathogens detection.
