In vivo tracing of human amniotic mesenchymal stem cells labeled with PKH26 in rat intrauterine adhesions model.

Author: Yuan YANG ¹ ; Yanhua MAO ¹ ; Jia WANG ¹ ; Congcong SUN ¹ ; Yingfeng ZHANG ¹ ; Xinpei CHEN ¹
Author Information

1. Obstetrics and Gynecology Departement, the University-Town Hospital, Chongqing Medical University, Chongqing 401331, China.
Publication Type:Journal Article
Keywords: PKH26; human amniotic mesenchymal stem cells (hAMSCs); intrauterine adhesions (IUA); trance
From:Chinese Journal of Biotechnology 2018;34(10):1660-1667
CountryChina
Language:Chinese
Abstract: To observe the migration of human amniotic mesenchymal stem cells (hAMSCs) labeled with PKH26 in the endometrium of rats intrauterine adhesion. hAMSCs were isolated, identified and labeled with PKH26 to detect the biological characteristics of the cells. Rat intrauterine adhesion models were established using mechanical and infective method and PKH26-labeled hAMSCs were transplanted through the tail vein. The distribution of PKH26 labeled hAMSCs in the endometrium of rats were observed with the fluorescence confocal microscope. The results showed that PKH26 stain had no significant effect on cell activity, cycle, apoptosis and so on. PKH26-labeled positive cells were mainly distributed in injured endometrium of rats. It shows that the PKH26 labeling technique is a safe and effective method for tracing the human amniotic mesenchymal stem cells in the treatment of intrauterine adhesions.