Chinese herbal medicine Euphorbia esula extract induces apoptosis and inhibits the proliferation, migration and invasion of multidrug resistant gastric carcinoma cells.
10.7507/1001-5515.201609009
- Author:
Xianli GUO
1
;
Zhaoying FU
2
,
3
;
Yun BI
4
;
Jun ZHENG
5
;
Lei WANG
5
;
Xiaolong HE
5
;
Fei LI
5
;
Xing LEI
5
;
Qingquan REN
5
Author Information
1. Department of Biochemistry and Molecular Biology, Yan'an University, Yan'an, Shaanxi 716000, P.R.China.
2. Department of Biochemistry and Molecular Biology, Yan'an University, Yan'an, Shaanxi 716000, P.R.China
3. Institute of Molecular Biology and Immunology, Yan'an University, Yan'an, Shaanxi 716000, P.R.China.
4. First Affiliated Hospital, Yan'an University, Yan'an, Shaanxi 716000, P.R.China.13409110833@163.com.
5. First Affiliated Hospital, Yan'an University, Yan'an, Shaanxi 716000, P.R.China.
- Publication Type:Journal Article
- Keywords:
Euphorbia esula;
cell apoptosis;
cell invasion;
cell migration;
cell proliferation;
gastric cancer;
multidrug resistance
- From:
Journal of Biomedical Engineering
2018;35(2):244-251
- CountryChina
- Language:Chinese
-
Abstract:
This paper aims to study the effects of traditional Chinese medicine on multidrug resistant human gastric cancer cells in the cell proliferation, migration, invasion and apoptosis, and to study the apoptosis-inducing pathway. Different dilutions of extract were used to process human multidrug resistant gastric cancer SGC7901/ADR cells. Cell proliferation inhibition phenomenon was determined by MTT experiment. Nuclear morphological changes of apoptotic cells and apoptotic indexes were observed and determined by Hochest33528 staining followed with fluorescence microscope observing. Flow cytometry was used to detect cell apoptosis rate. Cell migration and invasion ability were observed and determined by Transwell method. Spectrophotometry was used to detect caspase-3 and caspase-9 enzyme activity. Western blotting was used to detect subcellular distribution of cytochrome c. The results showed that extract had obvious inhibition effect on proliferation of gastric cancer multidrug resistant SGC7901/ADR cells, which was time- and concentration-dependent. After processing multidrug resistant gastric cancer SGC7901/ADR cells with extract, the apoptotic index and apoptosis rate were significantly increased than those in the control group, which showed a time- and dose-dependent mode; but if a caspase inhibitor was added, apoptosis index was not obviously increased. Transwell method showed that migration and invasion ability of the extract-processed SGC7901/ADR cells dropped significantly. Spectrophotometry showed that in extract-processed SGC7901/ADR cells, caspase-3 and caspase-9 expression were increased, which had significant differences with the control group. Western blotting test showed that the distribution of cytochrome c decreased in mitochondria, while increased in the cytoplasm (i.e., cytochrome c escaped from mitochondria to the cytoplasm). In conclusion, extract could inhibit the proliferation, migration and invasion, and induce apoptosis in human gastric cancer multidrug resistant SGC7901/ADR cells; and cytochrome c, caspase-9 and caspase-3 might be involved in cell apoptosis induced by extract, suggesting endogenous or mitochondrial apoptotic pathway.
