Preparation and properties of paclitaxel-loaded self-assembling nano-micelles of cholesterol-bearing γ-Polyglutamic acid.
10.7507/1001-5515.201708027
- Author:
Fan HU
1
;
Gang XIAO
2
;
Yuchuan WANG
2
;
Jun YAO
3
;
Xin CAO
2
Author Information
1. Analysis and Test Center of Nanjing Medical University, Nanjing 211166, P.R. China.
2. School of Basic Medical Science, Department of Biotechnology, Nanjing Medical University, Nanjing 211166, P.R.China.
3. School of Basic Medical Science, Department of Biotechnology, Nanjing Medical University, Nanjing 211166, P.R.China.joelyao@njmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
drug carrier;
nano-micelles;
pharmacokinetics;
tumor inhibiting test;
γ-polyglutamic acid
- From:
Journal of Biomedical Engineering
2018;35(3):403-408
- CountryChina
- Language:Chinese
-
Abstract:
Paclitaxel (PTX)-loaded self-assembling nano-micelles (PTX/NMs) were prepared based on amphiphilic cholesterol-bearing γ-polyglutamic acid (γ-PGA-graft-CH). The properties of PTX/NMs and were investigated. The results indicated that PTX could be entrapped in -PGA-graft-CH NMs. PTX/NMs was characterized with a size of (343.5 ± 7.3) nm, drug loading content of 26.9% ± 0.8% and entrapment efficiency of 88.6% ± 1.7% at the optimized drug/carrier ratio of 1/10, and showed a pH-sensitive sustainable drug-release and less cytotoxicity . release and the pharmacokinetics study in mice showed that the elimination half-life ( ) and area under curve (AUC) of PTX/NMs were significantly higher than those of PTX/polyoxyethylene castor oil (PTX/PCO), and less clearance (CL) of PTX/NMs was also observed. PTX/NMs were distributed higher in liver and tumor than PTX/PCO, and showed a good tumor-inhibiting activity in tumor-bearing mice. This study would lay a foundation on the potential application of -PGA-graft-CH NMs were the antitumor drug-delivery.
