Effect of sanguinarine on biomechanics of rat airway smooth muscle cells.
10.7507/1001-5515.201708025
- Author:
Mingzhi LUO
1
;
Peili YU
1
;
Yang JIN
2
;
Lei LIU
1
;
Jingjing LI
1
;
Yan PAN
1
;
Linhong DENG
3
Author Information
1. Changzhou Key Laboratory of Respiratory Medical Engineering, Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, Jiangsu 213164, P.R.China.
2. Bioengineering College, Chongqing University, Chongqing 400044, P.R.China.
3. Changzhou Key Laboratory of Respiratory Medical Engineering, Institute of Biomedical Engineering and Health Sciences, Changzhou University, Changzhou, Jiangsu 213164, P.R.China.dlh@cczu.edu.cn.
- Publication Type:Journal Article
- Keywords:
airway smooth muscle cells;
biomechanics;
cell stiffness;
cell traction force;
sanguinarine
- From:
Journal of Biomedical Engineering
2018;35(4):583-591
- CountryChina
- Language:Chinese
-
Abstract:
This study aimed to evaluate the effect of sanguinarine on biomechanical properties of rat airway smooth muscle cells (rASMCs) including stiffness, traction force and cytoskeletal stress fiber organization. To do so, rASMCs cultured were treated with sanguinarine solution at different concentrations (0.005~5 μmol/L) for 12 h, 24 h, 36 h, and 48 h, respectively. Subsequently, the cells were tested for their viability, stiffness, traction force, migration and microfilament distribution by using methylthiazolyldiphenyl-tetrazolium bromide assay, optical magnetic twisting cytometry, Fourier transform traction microscopy, scratch wound healing method, and immunofluorescence microscopy, respectively. The results showed that at concentration below 0.5 μmol/L sanguinarine had no effect on cell viability, but caused dose and time dependent effect on cell biomechanics. Specifically, rASMCs treated with sanguinarine at 0.05 μmol/L and 0.5 μmol/L for 12 and 24 h exhibited significant reduction in stiffness, traction force and migration speed, together with disorganization of the cytoskeletal stress fibers. Considering the essential role of airway smooth muscle cells (ASMCs) biomechanics in the airway hyperresponsiveness (AHR) of asthma, these findings suggest that sanguinarine may ameliorate AHR via alteration of ASMCs biomechanical properties, thus providing a novel approach for asthma drug development.
