Research progress of producing genetically modified pigs by CRISPR/Cas9 in the medical field.
10.7507/1001-5515.201802046
- Author:
Mengyu GAO
1
;
Guang YANG
2
;
Ji BAO
1
Author Information
1. Laboratory of Pathology, Key Laboratory of Transplant Engineering and Immunology, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
2. Experimental Animal Center, West China Hospital, Sichuan University, Chengdu 610041, P.R.China.
- Publication Type:Journal Article
- Keywords:
clustered regularly interspersed short palindromic repeat/CRISPR-associated 9;
gene editing;
genetically modified pig;
large animal disease models
- From:
Journal of Biomedical Engineering
2018;35(4):637-642
- CountryChina
- Language:Chinese
-
Abstract:
As pigs are similar to humans in anatomy, physiology and pathology, nutrition metabolism and disease characteristics, genetically modified pigs are already used for the studies of disease mechanism, pathology and toxicology and the evaluation of drugs. But the production of large modified animals is difficult, cumbersome, time-consuming and costly. With the breakthrough of gene editing technology, clustered regularly interspersed short palindromic repeat (CRISPR)/CRISPR-associated 9( Cas9)(CRISPR/Cas9) technology has greatly improved the mutation efficiency, reduced the cost and simplified the steps, and promoted the widespread application of genetically modified pigs. In this paper, the production methods of genetically modified pigs and the research progress of genetically modified pigs by CRISPR/Cas9 in the medical field were reviewed.
