Study on biocompatibility of carbon-based composites.
10.7507/1001-5515.201708014
- Author:
Yanxiong YIN
1
;
Shu YU
2
;
Yunping LI
1
;
Qiang WU
1
;
Xiao LI
1
;
Hui ZHONG
3
;
Youwen DENG
4
;
Tao XIAO
4
;
Lihong LIU
4
;
Xiaoning GUO
4
Author Information
1. Key Laboratory for Powder Metallurgy, Central South University, Changsha 410083, P.R.China.
2. Key Laboratory for Powder Metallurgy, Central South University, Changsha 410083, P.R.China.yushu@csu.edu.cn.
3. School of Life Sciences, Central South University, Changsha 410013, P.R.China.
4. Second Xiangya Hospital, Central South University, Changsha 410011, P.R.China.
- Publication Type:Journal Article
- Keywords:
biocompatibility;
carbon-based composites;
particles shedding
- From:
Journal of Biomedical Engineering
2018;35(5):740-748
- CountryChina
- Language:Chinese
-
Abstract:
Silicon carbide (SiC) film and silicon dioxide (SiO ) film were deposited on the surface of carbon/carbon composite (C/C) by low pressure chemical vapor deposition (LPCVD). The biocompatibility of the three carbon-based composites, e. g. C/C, C/C-SiC, C/C-SiO were investigated by cytotoxicity test, cell direct contact and cell adhesion experiments. Cytotoxicity, cell direct contact and cell adhesion showed that the three materials had no toxic effect on mouse fibroblasts (L929 cells). However, the particles dropped off from the three materials had a great impact on evaluation accuracy of the thiazolyl blue (MTT) test. More the particles were lost, more growth inhibition to L929 cells. The evaluation accuracy of MTT method can be kept with the filtered extract of materials. Furthermore, the results of surface particles shedding experiment showed that the amount of surface particles shed from C/C-SiO was the most, followed by C/C and C/C-SiC in 72 hours. Particles shedding curves showed there was a peak reached at eighth hour and then declined to the thirty-sixth hour. The filtrate analysis showed that there was no ion exchange between the three materials and simulated body fluid (SBF) solution. The results of this study on biocompatibility of carbon-based composites have certain guiding significance for their future application in clinical filed.
