Analysis on ecological factors and active components content of wild Dipsacus asper in Chongqing Wulong district.
10.19540/j.cnki.cjcmm.20180404.002
- Author:
Yuan YUE
1
;
Yong-Sheng WEI
1
;
Cong YAO
2
;
Yi-Quan ZHOU
1
;
Chang-Hua WANG
1
;
Rui PAN
1
;
Xian-You QU
1
Author Information
1. Chongqing Academy of Chinese Materia Medica, Chongqing 400065, China.
2. Chongqing Key Laboratory of Traditional Chinese Medicine Resources, Chongqing 400065, China.
- Publication Type:Journal Article
- Keywords:
Dipsacus asperoides;
determination of content;
ecological factors;
high performance liquid chromatography(HPLC)
- From:
China Journal of Chinese Materia Medica
2018;43(13):2740-2746
- CountryChina
- Language:Chinese
-
Abstract:
An HPLC method was developed for the determination of iridoid glycosides (loganin acid, loganin, sweroside) and saponins (asperosaponin Ⅵ) in the wild Dipsacus asper. A total of 108 samples consecutive growing 12 month were collected in 9 plots in Wulong district of Chongqing. Subsequent analysis of the content of loganin acid, loganin, sweroside and asperosaponin Ⅵ was performed by HPLC to evaluate the quality. In addition, 20 climate data provided by the world climate database (http://www.worldclim.org/) was analyzed to deduce the correlation between the growing environment factors and the active ingredient content accumulation of D. asperoides and choose the apposite growing environment for D. asper. The range of active ingredient content in wild D. asper were 0.01%-3.80%(loganin acid), 0.08%-0.62%(loganin), 0.12%-0.78%(sweroside), 0.64%-5.26%(asperosaponin Ⅵ). The highest content of these active ingredients was concentrated from February to April, with 2.64% of loganin acid, 0.36% of loganin), 0.57% of sweroside, and 3.09% of asperosaponin Ⅵ. The method used for determination of the active ingredient content in D. asper was simple and convenient with accurate result. The selection of the quadrats is scientific and reasonable and can be used for the analysis of the contents of the wild D. asper, thus provide a reference for quality evaluation of D. asper and protection of D. asper resources.
