Comparative study on contents of four alkaloids in homologous herbal medicines--Arecae Pericarpium and Arecae Semen.
10.19540/j.cnki.cjcmm.20180514.009
- Author:
Lian-Chao TIAN
1
;
Shao-Rong QIN
2
;
Hong YI
3
;
Chun LI
3
;
Hui-Wen MA
3
;
Cheng-Tao REN
1
;
Yun LI
3
;
Xiao-Qian LIU
3
;
Zhi-Min WANG
3
Author Information
1. School of Traditional Chinese Medicine， Guangdong Pharmaceutical University， Guangzhou 510006, China.
2. Taiji Group Chongqing Fuling Pharmaceuticals Co.， Ltd.， Chongqing 408000， China.
3. National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences， Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
Arecae Pericarpium;
Arecae Semen;
arecaidine;
arecoline;
guvacine;
guvacoline
- From:
China Journal of Chinese Materia Medica
2018;43(14):2850-2856
- CountryChina
- Language:Chinese
-
Abstract:
To establish a high performance liquid chromatography (HPLC) method for simultaneous determination of four alkaloids(arecoline， guvacoline， arecaidine， and guvacine) in Arecae Pericarpium (AP) and Arecae Semen (AS), and compare the contents of these four alkaloids between different medicinal parts. The chromatographic conditions were as follows:Welch SCX（4.6 mm×250 mm， 5 μm）column, with acetonitrile-0.2% phosphoric acid solution (adjusted to pH 3.85-3.90 with ammonium hydroxide) at 50:50 as the mobile phase, at a flow rate of 0.5 mL·min⁻¹. The column temperature was set at 35 °C, and the detection wavelength was 215 nm. The results of content determination in 7 batches of AS and 10 batches of AP showed that, the contents of 4 alkaloids in AS (arecaidine 0.020%-0.045%, guvacine 0.031%-0.086%, arecoline 0.194%-0.346%, and guvacoline 0.065%-0.094%) were generally higher than those in AP (arecaidine 0.10%-0.032%, guvacine 0.006%-0.029% arecoline 0.00%-0.070%, and guvacoline 0.00%-0.020%), and most of the APs had no arecoline and arecaidine at all in fruit peel. The above results indicated that different alkaloids can be used to distinguish the different medicinal parts of Arera catechu. Arecoline， guvacoline， arecaidine， and guvacine can be used as the quality control markers of AS, while for AP, only arecaidine and guvacine were needed.
