Study on quality control method of toad venom based on characteristic chromatogram and QAMS.
10.19540/j.cnki.cjcmm.2018.0088
- Author:
Peng-Fei WANG
1
;
Yun-Ge FANG
1
;
Zong-Yun LI
2
;
Liang-Mian CHEN
3
;
Zhi-Min WANG
3
;
Zhong-Mei ZOU
4
;
Hui-Min GAO
3
Author Information
1. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
2. Harrison International Peace Hospital, Hengshui 053000, China.
3. National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
4. Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193， China.
- Publication Type:Journal Article
- Keywords:
bufadienolides;
characteristic chromatogram;
quantitative analysis of multi-components by single marker (QAMS);
toad venom
- From:
China Journal of Chinese Materia Medica
2018;43(14):2863-2871
- CountryChina
- Language:Chinese
-
Abstract:
Toad venom (Chansu) is prepared from the dried secretion of parotid gland and skin gland from Bufo bufo gargarizans or B. melanostictus. Up to now, much attention shall be paid to the poor quality of commercial toad venom because of the adulteration. So, it is urgent to establish a scientific and perfect quality control method to improve the quality of toad venom and guarantee its safety and effectiveness in clinical application. The different batches of toad venom samples were assayed by high performance liquid chromatography (HPLC) and the quantitative analysis of multi-components by single marker (QAMS) was used to detect the contents of five bufagenins. As a result, the reference characteristic chromatogram was established, displaying serotonin, gamabufotalin, arenobufagin, hellebrigenin, telocinobufagin, bufotalin, cinobufotalin, bufalin, cinobufagin and resibufogenin as characteristic peaks. Taking cinobufagin as an internal reference substance, QAMS was verified for the determination of five bufagenins (gamabufotalin, bufotalin, bufalin, cinobufagin, resibufogenin) in toad venom samples. The durability and applicability of the relative correction factor (RCF) were also studied systematically. RCFs of cinobufagin to gamabufotalin, bufotalin, bufalin and resibufogenin were determined as 1.05, 0.895, 1.09 and 0.913, respectively. The characteristic chromatogram and QAMS established in this study could effectively control the quality of toad venom and provide scientific evidence for the improvement of the quality standard of the toad venom to be described in Chinese Pharmacopoeia (2020 edition).
