Structural identification of related substances in Breviscapine by UPLC-QTOF-MS.
10.19540/j.cnki.cjcmm.2018.0089
- Author:
Meng ZHAO
1
;
Peng-Fei WANG
2
;
Xiao-Mei WANG
2
;
Yuan LIU
2
;
Xiao-Qian LIU
2
;
Liang-Mian CHEN
2
;
Hui-Min GAO
2
;
Zhi-Min WANG
2
;
Wei ZHANG
3
Author Information
1. College of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450008, China.
2. National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences， Beijing 100700, China.
3. Kunming Longjin Pharmaceutical Co., Ltd., Kunming 650503, China.
- Publication Type:Journal Article
- Keywords:
HPLC;
UPLC-QTOF-MS;
breviscapine;
related substance;
structural identification
- From:
China Journal of Chinese Materia Medica
2018;43(14):2872-2877
- CountryChina
- Language:Chinese
-
Abstract:
To systematically identify the related substances in the original materials of breviscapine injection, 18 batches of samples collected from different pharmaceutical companies, its ethanol extract and breviscapine mother liquor concentrate were analyzed by high performance liquid chromatography (HPLC), and their structures were identified by ultra performance liquid chromatography and quadruple/time-of-flight mass spectrometry (UPLC-QTOF-MS). Under the selected chromatographic conditions, scutellarin and related substances have good resolution and 13 related substances were observed. Based on the molecular weight and fragmentation patterns obtained by UPLC-QTOF-MS as well as reference substances, their structures were elucidated as 6-hydroxyapigenin-6--glucosyl-7--glucuronide (1), 5,7,8,3',4',5'-hexahydroxyflavone-7--glucuronide (2), 5,6,7,3',4'-pentahydroxyflavone-7--glucuronide（3）and its isomer (4), patuletin-3--glucuronide (5), methoxylscutellarin (6), apigenin 7--glucuronide (7), isorhamnetin 7--glucuronide (8), diosmetin 7--glucuronide (9), scutellarein (10), scutellarin methyl ester (11), scutellarin ethyl ester (12), and apigenin (13). This study has clarified related substances in the original materials of breviscapine injection, providing references for the improvement of quality control for breviscapine drug material and its preparations.
