HPLC fingerprint of Zishen Yutai pills and simultaneous determination of 5 index components.
10.19540/j.cnki.cjcmm.2018.0090
- Author:
Hui-Wen MA
1
;
Qi ZOU
2
;
Chun LI
1
;
Zhen YIN
2
;
Hong YI
1
;
Lian-Chao TIAN
1
;
Cheng-Tao REN
1
;
Xiao-Qian LIU
1
;
Zhi-Min WANG
1
Author Information
1. National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
2. Guangzhou Baiyunshan Zhongyi Pharmaceutical Co., Ltd., Guangzhou 510530, China.
- Publication Type:Journal Article
- Keywords:
Zishen Yutai pills;
asperosaponin Ⅵ;
chlorogenic acid;
fingerprint;
loganic acid;
loganin;
sweroside
- From:
China Journal of Chinese Materia Medica
2018;43(14):2878-2883
- CountryChina
- Language:Chinese
-
Abstract:
To establish the HPLC fingerprint and determine five index components (loganic acid， chlorogenic acid， loganin， sweroside and asperosaponin Ⅵ) of Zishen Yutai pills by high performance liquid chromatography, and provide a scientific basis for its quality control. The fingerprint chromatogram was analysed by the chromatographic fingerprint similarity evaluation system for tradition Chinese medicine (2012), fifteen common peaks were obtained at the wavelength of 254 nm. Different batches of Zishen Yutai pills showed a similarity of above 0.90 in HPLC fingerprint profiles. For the quantitive analysis method, The separation of five components showed good regression (>0.999 2) with linear ranges, and the mean recoveries were in the range of 97.62%-101.9%, with the RSD (=9) less than 3%. The established fingerprint and quantitative analysis methods are highly specific, simple and accurate, which can reflect the quality of Zishen Yutai pills more comprehensively, and can be used for its quality control.
