Therapeutic potential of n-butanol extract of Pulsatilla decoction in a murine model of ulcerative colitis induced by DSS combined with Candida albicans colonization.
10.19540/j.cnki.cjcmm.20180417.001
- Author:
Xiao-Mei WANG
1
;
Ge TIAN
2
;
Qiang-Jun DUAN
2
;
Da-Qiang WU
2
;
Jing SHAO
2
;
Tian-Ming WANG
2
;
Chang-Zhong WANG
2
Author Information
1. School of Nursing, Anhui University of Chinese Medicine, Hefei 230012, China.
2. School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine, Hefei 230012, China.
- Publication Type:Journal Article
- Keywords:
Candida albicans;
n-butanol extract of Pulsatilla decoction;
ulcerative colitis
- From:
China Journal of Chinese Materia Medica
2018;43(14):2979-2984
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the mechanism of n-butanol extract of Pulsatilla decoction (BAEB) against murine ulcerative colitis (UC) model induced by DSS combined with Candida albicans (CA) colonization, mice were randomly divided into normal control group, DSS group, DSS+CA group, BAEB high, medium and low dose group, and positive drug Mesalazine group. The general condition of mice was observed, fungal loads of murine intestinal contents were detected by plate method, colonic pathological change of mice was examined by HE staining. ASCA in serum and IL-6, IL-8, IL-1β, HBD-2, HBD-3 in colonic mucosa were detected by ELISA. The results showed that, compared with DSS group, the general condition and ASCA in serum had no obvious change for DSS+CA group, but the fungal loads in intestinal contents, the colonic pathological damage, and the levels of IL-6, IL-8, IL-1β, HBD-2, HBD-3 in colonic mucosa were greater than that of DSS group. High dose of BAEB group and Mesalazine group could improve the colonic pathology, decrease IL-6, IL-8, IL-1β, HBD-2, HBD-3 expression level. In conclusion, BAEB could effectively improve the UC symptoms in mice induced by DSS combined with CA colonization, and inhibit the inflammatory factors such as IL-6, imply that BAEB is of important value for the treatment of intestinal fungal-related colitis.
