Study on quality control of Holotrichia diomphalia larvae by TLC and HPLC.
10.19540/j.cnki.cjcmm.2018.0044
- Author:
Yan-Tao SUN
1
;
Jing SUN
1
;
Lu-Hong WANG
2
;
Lei ZHAO
2
Author Information
1. College of Chemistry, Jilin Normal University, Siping 136000, China.
2. Siping Institute for food and Drug Control, Siping 136000, China.
- Publication Type:Journal Article
- Keywords:
HPLC;
Holotricha diomphalia larvae;
TLC;
determination;
guanosine;
inosine
- From:
China Journal of Chinese Materia Medica
2018;43(6):1189-1191
- CountryChina
- Language:Chinese
-
Abstract:
The TLC method was established for identification of Holotricha diomphalia larvae and the HPLC method was used to determine the content of inosine and guanosine in H. diomphalia larvae. The HPLC analysis was performed on a Waters HSS T3（4.6 mm×250 mm, 5 μm） column of with mobile phase consisting of acetonitrile (A) and 0.08% trifluoroacetic acid (B) in gradient elution. The detection wavelength was 260 nm. The flow rate was 1.0 mL·min⁻¹. The column temperature was 30 °C. As a result, TLC identification method had a good reproducibility and highly specificity. The linear equations of inosine and guanosine were in good linear range (r>0.999 8). The average recovery of inosine and guanosine was 96.53% (RSD=1.6%), 99.71% (RSD=2.7%). The method is simple, accurate and reproducible, which can provide a basis for quality standard improvement H. diomphalia larvae.
