Characterization of sediment of water extract of Guizhi decoction and its effect on relevant compound (fractions) in decoction.
10.19540/j.cnki.cjcmm.20180117.004
- Author:
Hai-Jian YUAN
1
;
Wei LI
1
;
Jian-Ming JIN
1
;
Xiao-Bin JIA
2
;
Tao ZHOU
1
;
Hui WANG
1
;
E SUN
2
;
Hui-Yan NI
3
Author Information
1. Taizhou Polytechnic College, Taizhou 225300, China.
2. Key Laboratory of New Drug Delivery Systems of Chinese Materia Medica, Nanjing 210028, China.
3. The Fourth People's Hospital of Zhenjiang, Zhenjiang 212001, China.
- Publication Type:Journal Article
- Keywords:
Guizhi decoction;
LC-MS;
chemical composition;
sediment
- From:
China Journal of Chinese Materia Medica
2018;43(8):1633-1641
- CountryChina
- Language:Chinese
-
Abstract:
In order to study the effect of sediment of water extract of Guizhi decoction on the stability, clarity and peaks area, and characterize the chemical composition of the sediments, HPLC and MS methods were established. Through comparison of the common peak areas and the turbidity value of water extract and filtrate, the sediments could greatly change the common peak areas of the decoction (for more than 5 times of the study standard); at the same time, the turbidity value of the decoction could increase by （38.66±1.57）% in 48 h [particularly by （24.54±1.68）% in 6 h]. The test indicated that the sediments had an effect on the stability and clarity under the test conditions in Guizhi decoction. The study confirmed that the sediments were mainly derived from Cassia twig, Paeonia lactiflora and Glycyrrhiza uralensis. On the basis of the reference information, the accurate molecular weight and fragment ion information provided by LC-MS were analyzed, the molecular formula of sediments components A-F were determined, and the possible structural information of components B, C, D and F were deduced. It was suggested that the multi-index, multi-target and multi-angle analysis could ensure the quality of traditional Chinese medicine and the effect of clinical medication. The study also suggested the effect of the sediments on clinical application and the preparation of traditional Chinese medicine.
