EFFECT OF TLR7 LIGAND ON SIGNAL TRANSDUCTION OF INTERFERON GAMMA
- VernacularTitle:TLR7 ЛИГАНД ИНТЕРФЕРОН ГАММА ДОХИО ДАМЖИЛТАНД НӨЛӨӨЛӨХ НЬ
- Author:
Baasansuren E
1
;
Javkhlan B
1
;
Baljinnyam T
1
;
Erkhembayar Sh
1
;
Batkhishig M
1
;
Dolgorsuren S
2
;
Enkhsaikhan L
2
;
Ulziisaikhan J
2
;
Khongorzul B
3
;
Baigalmaa B
3
;
Galindev B
3
;
Sodnomtsogt L
3
;
Nyambayar D
3
;
Nyamdorj D
4
;
Munkhtuvshin N
5
;
Munkhbat B
3
;
Bilegtsaikhan Ts
3
Author Information
1. Department of Microbiology and Immunology, School of Bio-Medicine, MNUMS
2. Department of Anatomy, SBS, MNUMS
3. Core Laboratory, Science Technology Center, MNUMS
4. Department of Anatomy, School of Bio-Medicine, MNUMS
5. Central Scientific Research Laboratory, Institute of Medical Sciences, Mongolia
- Publication Type:Journal Article
- Keywords:
IFN-y, nitric oxide, imiquimod, TLR7
- From:Innovation
2017;11(4):14-17
- CountryMongolia
- Language:Mongolian
-
Abstract:
BACKGROUND: Toll like receptors (TLRs) are a class of proteins that key role in the innate immune system. TLR7 is expressed on monocytes, macrophages and dendritic cells, T cell, B cell and eosinophiles. TLR7, originally identified as recognizing imidaquinoline, loxibrine, broprimine and ssRNA, ssRNA viruses such as vesicular stomatitis virus, influenza A virus and human immunodefiency virus. It is known that virus ssRNA affects signaling molecule of IFN-y. Objective: To determine gene and protein activation of IFN-y signal transduction by TLR7 ligand in the endothelial cells.
MATERIAL: In study we used mouse aortic linear endothelial cell which is cultured (END-D) in 5% heat- inactivated fetal calf serum (FCS), medium (DMEM) containing antibiotic mix(penicillin G, streptomycin, amphotericin B) at 37°C (5% CO2). Endothelial cells treated with synthetic IFN-γ and imiquimodligands, then the NO (nitric oxide) concentration in the supernatant is determined by Griess reagent. Endothelial cells are cultured in 6 well cell culture plate and in each well 2*104cells are expected to be grown for 24 hours of culture. Then, the cells are treated with synthetic IFN-γ and имиквимод ligand for 6 hours and the NO signaling gene activation iNOS mRNA expression which is induced by IFN-γ is determined by RT-qPCR. Endothelial cells are cultured in 12 well cell culture plate and in each well 2*104 cells are expected to be grown for 18 hours of culture. Then, the cells are treated with synthetic IFN-γ and imiquimodligands for 24 hours and the NO signaling protein iNOS expression which is induced by IFN-γ is determined by western blotting. The experiment was conducted as representation mean of at least three test results. The difference between statistical probabilities is determined by the “Students” t test. The p<0.01 value is assumed to be statistically different.
RESULTS: TLR7 ligand imiquimodaugmented interferon gamma induced nitric oxide production TLR7 ligand imiquimodincreased interferon gamma induced iNOS mRNA gene expression. TLR7 ilgand imiquimodup-regulated interferon gamma induced iNOS protein expression.
CONCLUSIONS: TLR7 ligand imiquimod augments IFN-γ signaling in the endothelial cells. This synergistic effect has revealed in the levels of gene and protein expression.
