Determination of phthalate esters in physiological saline solution by monolithic silica spin column extraction method
- VernacularTitle:Determination of phthalate esters in physiological saline solution by monolithic silica spin column extraction method
- Author:
Lu LU
;
Yuki HASHI
;
Zhihua WANG
;
Yuan MA
;
Ming LIN
;
- Publication Type:Journal Article
- Keywords:
monolithic silica spin column;
phthalate esters;
physiological saline samples;
ultra fast liquid chromatography- mass spectrometry （UFLC-MS）
- From:
Journal of Pharmaceutical Analysis
2011;01(2):92-99
- CountryChina
- Language:Chinese
-
Abstract:
Monolithic silica spin column extraction （MonoSpin-SPE） was developed as a simple, sensitive, and eco-friendly pretreatment method which combined with ultra-fast liquid chromatography-mass spectrometry （UFLC-MS） to determine the levels of six phthalate esters, dimethyl- （DMP）, diethyl- （DEP）, dipropyl- [ DPrP], butyl-benzyl- （BBP）, dicyclohexyl- （DcHP）, and di-n-octyl-（DOP） phthalate in physiological saline samples. Under optimized experimental conditions, the method was linear in the following ranges： 0.2- 50 μg/L for DMP, DEP, DPrP, DcHP and DOP; 5- 100 μg/L for BBP. The correlation coefficients （R2 ） were in the range of 0. 9951 - 0. 9995 for all the analytes and the limits of detection （LODs） and limits of quantification （LOQs） were in the ranges of 0.02 - 0.9 μg/L and 0.08 - 2.7μg/L, respectively. The pretreatment process showed good reproducibility with inter-day and intra-day relative standard deviations （RSDs） below 8.5% and 11.2%, respectively. This method was used to determine the levels of six phthalate esters in physiological saline samples and the recoveries ranged from 71.2% to 107.3%. DMP and DEP were found in actual physical saline samples （brand A and brand B）.
