The resistant phenotype and resistance gene detection of yak-derived Escherichia coli in Tibet
10.16303/j.cnki.1005-4545.2017.08.12
- VernacularTitle:西藏牦牛源大肠杆菌主要耐药表型及耐药基因检测
- Author:
Sizhu SUOLANG
;
Gang WANG
;
Run-bo LUO
;
Ga GONG
;
Cuomu YIXI
;
- Keywords:
Tibetan yak;
E.coli;
rmtB;
flor;
sul
- From:
Chinese Journal of Veterinary Science
2017;37(8):1501-1506
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to understand the Escherichia coli drug resistance isolated from different parts of Tibet yak in order to provide scientific evidence for controling Escherichia coli.PCR analysis of drug resistance phenotype and drug resistance gene of aminoglycosides,florfenicol and sulfonamides were carried out in 200 strains of yak-derived Escherichia coli from 6 different places in Tibet.The results showed that the resistance rate of aminoglycoside to yak-derived Escherichia coli was 96％ of amikacin,94.59％ of streptomycin,19％ of neomycin,23％ of gentamicin,19％ of kanamycin.The detection rate of rmtB gene was 100％.The drug resistance rate of florfenicol was 25％ with 25％ detection rate of resistance geneflor.The resistance rate of sulfonamides was 32％ with 7％,7％ and 17％ detection rates of sul1,sul2 and sul3 respectively genes,Moreover,the result of drug resistance phenotype was consistent with that of drug resistance gene.Meanwhile,the expression of sull gene in different regions of Tibet were different in drug resistance of the bacilli,and those from the population-intensive and flow-sensitive Lhasa,Nyingchi and Xigaze were more resistant to drug resistance,while those from Ali,Shannan and Nagqu were relatively light.The above-mentioned drug-resistant phenotype and drug resistance genes were detected in different cities and regions of Tibet,and there were multiple drug-resistant phenomena.The results showed that there was resistance to Escherichia coli in Tibetan yak,which should be paid attention to,and suggested that the in Tibetan area the antibiotics should be rationally used to reduce the drug resistance.
