Differential proteomic analysis of T lymphocyte in mice immunized with Echinoccocus granulosus′14-3-3 recombinant vaccine
10.3969/j.issn.1673-4130.2015.23.010
- VernacularTitle:细粒棘球蚴14-3-3重组疫苗免疫小鼠T淋巴细胞差异蛋白质组分析
- Author:
Zongji LI
;
Wei ZHAO
;
- Publication Type:Journal Article
- Keywords:
Echinoccocus granulosus;
14-3-3 recombined vaccine;
T lymphocyte;
differencial proteome
- From:
International Journal of Laboratory Medicine
2015;(23):3388-3389,3392
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differentially expressed proteomics on T lymphocyte in mice immunized with 14‐3‐3 recom‐bined vaccine and injected with phosphate buffer solution (PBS) .Methods Mice immunized with 14‐3‐3 recombined vaccine were separated in the experiment group and those injected with PBS were selected in the control group .Two weeks after immunization with 14‐3‐3 recombined vaccine ,total protein of T lymphocyte isolated from the two groups was collected and separated by using the two dimensional electrophoresis(2‐DE) .The differentially expressed proteomics were compared .Results The 2‐DE map shown 11 differentially expressed protein plots ,including 7 protein plots spotted in the 2‐DE map of T lymphocyte from 14‐3‐3 recombined vaccine immunized‐mice and 4 protein plots spotted in the 2‐DE map of T lymphocyte from PBS injected‐mice .Conclusion There are differentially expressed proteomics of T lymphocyte in PBS injected‐mice and 14‐3‐3 recombined vaccine immunized‐mice ,which could contribute to clarifing the mechanism on protective immunity of 14‐3‐3 recombined vaccine and optimizing vaccine strategy for improving vaccine effect .
