Serum specific biomarkers for detection of lymphoma using SELDI-TOF-MS
10.3760/cma.j.issn.1009-9921.2011.11.010
- VernacularTitle:应用表面增强激光解吸电离-飞行时间-质谱技术筛选淋巴瘤患者血清特异性生物标志物
- Author:
Shu-ling HOU
;
Qiao-hua ZHANG
;
Jun WANG
;
Jian-xia HE
;
Su-tang GUO
;
- Publication Type:Journal Article
- Keywords:
Lymphoma;
Spectrometry,mass,matrix-assisted laser desorption-ionization;
Serum;
Proteomic patterns;
Biomarker
- From:
Journal of Leukemia & Lymphoma
2011;20(11):672-675,679
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify serum specific protein biomarkers for the early diagnosis of lymphoma patients by using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS)protein chip array.MethodsSELDI-TOF-MS technique and weak cation exchanger (WCX2)protein chip were used to detect the serum proteomic patterns of 96 patients with lymphoma (86 patients with non-Hodgkin lymphoma and 10 patients with Hodgkin lymphoma)and 30 normal control.The serum level of LDH were measured by biochemical methods,the serum level of β2-MG and CA125 were detected by enzyme-linked immunosorbent assay (ELISA).ResultsFour protein peaks pattern mass/charge ratio (M/Z) 6880,8564,8692 and 13751 were decreased in lymphoma patients,and they could be used to distinguish lymphoma from healthy people and other malignant tumors.Their sensitivity and specificity were 82.29 ％ and 80.00 ％,78.13 ％ and 73.30 ％,75.00 ％ and 80.00 ％,71.88 ％ and 83.30 ％ respectively in lymphoma patients.When it was used to early diagnosing lymphoma in stage Ⅰ or Ⅱ,the sensitivity of four protein peaks pattern (M/Z 6880,8564,8692 and 13 751) were 77.55 ％,71.43 ％,71.43 ％ and 67.35 ％respectively and the specificity of all four protein peaks pattern were 100.00 ％.Four protein peaks pattern sensitivity were higher than LDH,CA125 and β2-MG respectively.ConclusionApplication of SELDI-TOF-MS can be of great potential for the early diagnosis of lymphoma patients.
