Evaluation on effect of fluorescent staining and Ziehl-Neelsen staining methods for detecting Mycobacterium tuberculosis
10.3969/j.issn.1673-4130.2015.06.011
- VernacularTitle:荧光染色法与萋-尼染色法检测结核分枝杆菌的效果评价
- Author:
Xin LI
;
Qing QING
;
Duofu LI
;
- Publication Type:Journal Article
- Keywords:
microscope;
light-emitting diode;
fluorescence staining;
acid fast staining;
Mycobacterium tuberculosis
- From:
International Journal of Laboratory Medicine
2015;(6):745-746,748
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the results difference between the fluorescence staining and the acid fast staining (Ziehi‐Neelsen staining) methods in the detection of Mycobacterium tuberculosis ,and to compare the effects of the methylene blue solu‐tion ,Haris hematoxylin solution and potassium permanganate liquid as the redyeing reagents of the fluorescence staining method . Methods 198 sputum specimens collected from the patients with suspected tuberculosis symptoms and were performed the Ziehi‐Neelsen staining and the fluorescence staining respectively For comparing the difference in the detecting rate of Mycobacterium tu‐berculosis between the two kinds of method .The fluorescence staining adopted 0 .3% methylene blue solution ,0 .5% Haris hema‐toxylin solution and 0 .5% potassium permanganate solution as the redyeing reagents for comparing the effects of the fluorescence microscopic examination among different redying reagents .Results The detection rate of Mycobacterium tuberculosis was 66 .67%(132/198) for the Ziehi‐Neelsen staining ,94 .9% (188/198) for the fluorescence stainings and 94 .95% (188/198) for the methyl‐ene blue staining ,in which the detection rate of methylene blue redyeing was 94 .95% ,which of hematoxylin redyeing was 94 .44%(187/198) and which of potassium permanganate redyeing was 94 .44 (187/198) ,the differences among them were statistically sig‐nificant(P< 0 .05) .Conclusion The fluorescent staining method has the higher positive detection rate of Mycobacterium tubercu‐losis than the Ziehl‐Neelsen staining method ,in which 0 .3% methylene blue solution is a good background quenching redyeing solu‐tion .
