Analysis on fibrinogen detection by using PT-der assay and Von-Clauss assay on Sysmex CA-1500 Automated Coagulation Analyzer
10.3969/j.issn.1673-4130.2014.10.045
- VernacularTitle:Sysmex CA-1500全自动血凝仪PT-der法和Von-Clauss法检测纤维蛋白原的分析
- Author:
Zhenjie LIU
;
Yanhong HE
;
Ning XU
;
Wenjun HE
;
Zi′an WU
;
Liying HUANG
;
Tao LI
;
- Publication Type:Journal Article
- Keywords:
fibrinogen;
Von-Clauss assay;
PT-der assay;
linear regression
- From:
International Journal of Laboratory Medicine
2014;(10):1333-1334,1370
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the relevance and accuracy of fibrinogen (Fib) detection by using PT-der assay and Von-Clauss assay on Sysmex CA-1500 Automated Coagulation Analyzer .Methods PT-der assay and Von-Clauss assay on Sysmex CA-1500 Automated Coagulation Analyzer were employed to detect the plasma Fib concentrations of 755 blood samples .The dilution ratios of samples with high Fib concentration were 1∶8 ,2∶7 ,3∶6 ,4∶5 ,5∶4 ,6∶3 ,respectively .The dilution ratios were served as the abscissa ,and the Fib concentrations measured by two methods as the ordinate ,a simple linear regression analysis was per-formed .Results When Fib concentration was in 2 .0- <6 .0 g/L ,the Fib value obtained by Von-Clauss assay was higher than that by PT-der assay(P<0 .01) .When Fib concentration was below 2 .0 g/L or above 6 .0 g/L ,the Fib value obtained by PT-der assay was higher than that by Von-Clauss assay(P<0 .01) .The linear regression equations of PT-der assay and Von-Clauss assay were Y=4 .537 7X+1 .551 3(R2 =0 .897 3) ,Y = 7 .792 2X+ 0 .290 0(R2 =0 .980 5) ,respectively .Conclusion Von-Clauss assay can better reflect the Fib level of human body which has a blood clotting function .
