Cytolytic Activity Analysis of Grass Carp Perforin C-terminal Peptide
- VernacularTitle:草鱼穿孔素C端溶细胞活性分析
- Author:
Yu-Jie XIA
;
Chu-Xin WU
;
Cheng-Yu HU
;
- Publication Type:Journal Article
- Keywords:
Perforin C2 Cytolytic Grass carp
- From:
China Biotechnology
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
The cytolytic effect of perforin is a mechanism of anti-virus,killing microbial-infected cells and tumor cells.Perforin is a very important non-specific immune factors in fish.In order to understand the function of perforin,the cDNA of grass carp perforin C-terminal peptide was amplified from grass carp liver and kidney cDNA library.It contains a protein kinase C conserved region 2(C2).The cDNA was connected with pET32a,and transformed to expression bacteria DE3.PFP-C was expressed by a prokaryotic expression system and then purified by affinity chromatography.It showed a significant haemolytic activity when tested with rabbit red cells,the optimal pH for haemolytic activity was 7.5,and its haemolytic function dependents on Ca2+ apparently.
