Research on Alliinase Immobilization and Its Properties
- VernacularTitle:蒜氨酸酶的固定化及其酶学性质研究
- Author:
Jian-Qin ZHOU
;
Jian-Wen WANG
;
- Publication Type:Journal Article
- Keywords:
Alliinase Immobilization Enzyme properties
- From:
China Biotechnology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Alliinase is immobilized on pre-activated chitosan microspheres by glutaraldehyde.The optimum immobilization conditions are as follows: glutaraldehyde concentration is 4%,alliinase concentration 20.2U,immobilization time 2h.For immobilized alliinase,the highest activity was allowed at pH 7.0 and temperature 35℃,and the Michaelis constant(Km)was disclosed to be 7.9mmol/L by Lineweaver-Burk plot,and after 10 times reuses the immobilized alliinase lost no more than 10% activity.
