Large Protein Production Through Split Intein-Mediated Trans-Splicing
- VernacularTitle:通过断裂内含肽介导的反式剪接合成大的蛋白
- Author:
Jing ZHANG
;
Huan LIU
;
Jing ZHOU
;
Jian-Hua LIU
;
- Publication Type:Journal Article
- Keywords:
Large cytotoxic or membrane proteins Npu DnaE intein T7 RNA polymerase
- From:
China Biotechnology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Npu DnaE intein was used to produce some large proteins,which were difficult to obtain through conventional expression systems.A T7 expression system was described,by which the gene of T7 RNA polymerase is split into two pieces,and each piece fuses with Npu DnaE N-and C-terminal sequences respectively.Functional T7 RNA polymerase is created by mixing the two kinds of fusion constructs in vitro.The approach of split intein-mediated production of large proteins,in theory,readily generalizable to the purification of other large,cytotoxic or membrane proteins.
