Application of Tn5 Transposon Mutagenesis Technology in Molecular and Genetic Researches of Gram-negative Bacteria
- VernacularTitle:Tn5转座突变技术在革兰氏阴性细菌分子遗传研究中的应用
- Author:
Hong-Juan NIAN
;
Li-Mei CHEN
;
Kun-Zhi LI
;
- Publication Type:Journal Article
- Keywords:
Tn5 transposon mutagenesis Biocontrol mechanism Bacterial essential gene Virulence gene Metabolism regulatory gene Bacteria improvements
- From:
China Biotechnology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
With development of wide-host-range vector systems,Tn5 transposon and its derivative vectors have been widely applied to genetic research of gram-negative bacteria.The applications of Tn5 transposon mutagenesis technology to genetic researches of bacteria were briefly discussed,including researches on biological control mechanisms of biocontrol bacteria,identification of bacterial essential genes,discovering virulence genes of bacterial pathogens,characterization of metabolism regulatory genes and genetic improvements of bacteria.
