Development of a Reverse Dot Blot System for Rapid Detection of HBV Genotypes
- VernacularTitle:反向斑点杂交法快速检测HBV基因型反应条件的优化和建立
- Author:
Li ZHAO
;
Wen-Lu ZHANG
;
Yuan HU
;
Yan-Chen LIU
;
Guo-Qi LAI
;
Feng YANG
;
Ai-Long HUANG
;
- Publication Type:Journal Article
- Keywords:
Reverse dot blot Hepatitis B virus Genotypes Clinical specimen
- From:
China Biotechnology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
To develop a reverse dot blot assay for rapid detection of HBV genotypes.Specific oligonucleotides probes were desighed and immobilized on nylon membranes.The DNA sample to be tested was PCR-amplified with DIG labeling primers and then hybridized with the immobilized probes.This procedure for detecting HBV genotypes was simple,rapid and specificity.30 specimens in Chongqing area were collected and detected by this method,and results were evaluated using direct sequencing.Results showed that: This new method was applicable to precise detection HBV genotypes for specimen with copies up to 103,and the HBV genotyping results showed that genotype B was the predominant genotype in Chongqing area.
