N-terminal of ?-Synuclein Involved in Regulation of Mitochondrial Function
- VernacularTitle:?-突触核蛋白N-端结构域参与线粒体功能的调控
- Author:
Li LV
;
Tao ZHANG
;
Qi LIU
;
Chun-Xiang FAN
;
Ling ZHANG
;
Huan-Ying ZHAO
;
Chun-Li ZHAO
;
Hui YANG
;
- Publication Type:Journal Article
- Keywords:
N-terminal of ?-synuclein Mitochondria Mitochondrial membrane potential Cytochrome c
- From:
China Biotechnology
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To identify the functional domain of ?-Synuclein in affecting mitochondrial function and how the function to be impaired,especially,the mitochondrial membrane potential and the release of Cytochrome c.Methods: Harvest of ?-Syn-N and ?-Syn-△N by PCR,then subcloned into the pCMV-Myc mammalian expression vector.The recombinant plasmids were transfected into HEK293T cells by Lipofectamine 2000.After detecting the protein expression by Western blot,the functional domain was detected by co-immunoprecipitation.The mitochondrial membrane potential through flow cytometry and immunofluorescence,at the same time,the release of Cytochrome c through flow cytometry to detect.Results: The recombinant plasmids were constructed successfully.CO-IP has proved that N-terminal may be the functional domain of ?-Synuclein in affecting mitochondria.Over-expression of N-terminal could depolarize the mitochondrial membrane potential and induce the Cytochrome c releasing in MN9D cells.Conclusion: N-terminal may be the functional domain of ?-synuclein and over-expression of N-terminal could decrease mitochondrial activity.
