Identification and AHLs Detection of Dominant Bacterium of Sea Cucumber (Apostichopus japonic) Infected with the Skin Ulceration Syndrom
- VernacularTitle:仿刺参“腐皮综合症”病灶处优势菌的分离鉴定及AHLs信号分子的检测
- Author:
Ying ZHANG
;
Guo-Liang JIANG
;
Yun LIU
;
Zhi-Qiang WU
;
Hai-Yan MA
;
Ming-Xia SU
;
- Publication Type:Journal Article
- Keywords:
Apostichopus japonica,The skin ulceration disease,Dominant bacteria,Quorum sensing,Signal molecule
- From:
Microbiology
2008;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
The skin ulceration syndrome of sea cucumber is a kind of desease induced by bacterium.In order to investigate the bacterium of infected sea cucumber and detect the N-acyl-homoserine lactones(AHLs) se-cretion of the bacterium,7 bacterial strains were isolated from the infected sea cucumber.These strains were identified by physiological-biochemical characteristics and 16S rDNA sequence.Results show that strain C6 belongs to Tenacibaculum,strain 4 belongs to Shewanella putrefaciens group,strain TB belongs to Vibrio,strain BP2,BP3,BP4 and BP6 belong to Pseudoalteromonas,respectively.AHLs were detected with strain Agrobacterium tumefaciens KYC55.Among these bacterial strains,strain C6,4,TB,BP3 and BP4 can se-cret AHLs,while strain BP2 and BP6 can’t.And the AHLs activity differs,from the highest to the lowest are 4,TB,BP4,BP3 and C6.
