Application of Thermal Shift Assays in Purification of Recombinant TBEV Serine Protease
- VernacularTitle:Thermal Shift Assays在重组森林脑炎病毒丝氨酸蛋白酶纯化中的应用
- Author:
Jun-Ping ZHU
;
Dong-Ying FAN
;
- Publication Type:Journal Article
- Keywords:
Thermal shift assays Recombinant TBEV serine protease Purification
- From:
China Biotechnology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Thermal Shift Assays was applied for high-throughput screening optimized buffer system used in the gel filtration chromatography for purification of recombinant TBEV serine protease. The optimization included kinds of salt and pH. It was optimized new buffer remarkably improved the quality of the purified TBEV serine protease by increasing its monomer from 75% to 99%. This result may improve future crystal studies of this protein.
