Bridge PCR,An Easy Way for Concatemerizing DNA Tags
- VernacularTitle:桥式PCR,一种简易连接DNA标签序列的方法(英文)
- Author:
Jian-Ping MAO
;
Quan-Hui WANG
;
Ying ZHOU
;
Jing FANG
;
Yu-Fang CUI
;
- Publication Type:Journal Article
- Keywords:
Bioreactor Mouse embryonic stem cells Embryoid body Cardiomyocytes Differentiation
- From:
China Biotechnology
2006;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
In MAST (mRNA accessible site tagging),the DNA tags from synthesized library were employed for identifying mRNA accessible sites. A large number of tags were amplified and subcloned for sequencing to verify mRNA binding profiles. A PCR was designed by using one primer which bridges over the tag terminal sequences. In PCR reaction DNA tag fragments were concatemerized by a bridge primer in reaction cycles. The concatemerized tag fragments were subcloned and sequenced. Dozens of the concatemerized sequences contained thousands tags. The PCR was a simple,effective way which for sequencing tags in a high through put manner.
