Breeding and Fermentation Medium Optimization of Cold-active Cellulase Strain CNY086(Ⅰ)
- VernacularTitle:低温纤维素酶菌株CNY086选育及发酵培养基优化(Ⅰ)
- Author:
Liang CHEN
;
Nai-Yu CHI
;
Qing-Fang ZHANG
;
- Publication Type:Journal Article
- Keywords:
Cold-active cellulase, Breeding, Optimization
- From:
Microbiology
2008;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
21 cold-active strains which produced cold-active cellulase were isolated from Bohai Bay. One of the strains CNY01 was a kind of Trichoderma viride and its cellulase activity was 67.30 U/mL. Through UV and DES mutation, the strain CNY086 was bred and its cellulase activity was 92.17 U/mL. The strain CNY086 had stable ability to produce cellulase. Through orthogonal experiment, the optimal condition of the fermentation medium had been tested. The suitable medium was that straw power 1.20%, bran 0.70%, (NH4)2SO4 0.50%, KH2PO4 0.55%, in this condition the cellulase activity came to 108.55 U/mL.
