The Optimal Fermentation Conditions of Cold-active Cellulase Strain CNY086(Ⅱ)
- VernacularTitle:低温纤维素酶菌株CNY086发酵条件优化(Ⅱ)
- Author:
Liang CHEN
;
Nai-Yu CHI
;
Qing-Fang ZHANG
;
- Publication Type:Journal Article
- Keywords:
Cold active cellulase, Fermentation, Optimization
- From:
Microbiology
2008;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Based on the research of text (Ⅰ) which determined the fermentation medium, the fermentation condition of CNY086 including temperature, volume, inoculum and inoculum age had been studied by single factor and orthogonal experiment. The optimum temperature, liquid volume, inoculum and seed age were 15?C, 250 mL/500 mL, 15%, 10 h. Under the conditions above mentioned, enzyme activity reached 104.36 U/mL in 5 L fermentor.