Co-expression of PPV VP2 and LTB on the Surface of Lactobacillus casei
- VernacularTitle:猪细小病毒VP2与大肠杆菌不耐热肠毒素B亚单位在干酪乳杆菌表面共表达
- Author:
Xiang-Qing WANG
;
Di OU
;
An-Qi REN
;
Jun-Wei GE
;
Xin-Yuan QIAO
;
Li-Jie TANG
;
Yi-Jing LI
;
- Publication Type:Journal Article
- Keywords:
PPV, VP2 protein, LTB, Lactobacillus case, Co-expression
- From:
Microbiology
2008;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Lactobacillus casei was selected as an antigen delivery vehicle for the development of oral vaccine to express recombinant LTB and porcine parvovirus (PPV) VP2 protein. The fusion protein gene encoding PPV VP2 protein and LTB, was cloned into the surface expression vector pPG, and then the recombinant expression vector pPG-VP2-LTB was electrotransformed into Lactobacillus casei 393, generating recombinant strain pPG-VP2-LTB/L. casei 393. After induced by 2% Lactose in MRS broth, an about 78 kD protein was detected in the recombinant Lactobacillus casei by SDS-PAGE. The result of Western blot indicated that the protein possessed the antigenic specificity same as the native virus protein. The result of the whole bacteria cell ELISA indicated that the LTB protein was expressed at the same time. The results of indirect immunofluorescence test and immuno-gold electron microscopy showed that the interest protein was expressed on the surface of L. casei 393. The results provide potential for the development of lactic acid bacteria oral vaccine of PPV, which used LTB as mucosal adjuvant.
