Isolation and Identification of Marine Actinomycetes with Quorum Sensing Inhibitory Activity
- VernacularTitle:具有群体感应抑制活性海洋放线菌的分离和鉴定
- Author:
Wei-Shan WANG
;
Shan-Shan ZOU
;
Shou-Liang YIN
;
Qian-Hong GONG
;
Wen-Gong YU
;
- Publication Type:Journal Article
- Keywords:
Chromobacterium violaceum CV026,Marine actinomycetes,Quorum sensing inhibitory activity,Violacein,Phylogenetic analysis
- From:
Microbiology
2008;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Quorum sensing systems of pathogens are central regulators for the expression of virulence factors. Increasing evidence implies that targeting the quorum sensing system of many pathogenic bacteria is a promising therapeutic approach to control infections. In this work,we isolated 47 strains of actinomycetes from the mud sample of Jiaozhou Bay. Quorum sensing inhibitory activity was monitored by Chromobacterium violaceum CV026. As a result,the culture broth extract of actinomycetes WA-7 was found to have significant quorum sensing inhibitory activity. This strain was assigned to the genus Streptomyces based on its 16S rDNA sequence. Further investigation revealed that the extract could inhibit the quorum sensing-controlled violacein and proteases production of C. violaceum in a concentration-dependent manner.
