Screening and Identification of a New Elastase-producing Strain
- VernacularTitle:新的产弹性蛋白酶菌株的筛选与鉴定
- Author:
Shuang-Fa LIU
;
De-Rong AN
;
Li-Xia GOU
;
Na LI
;
- Publication Type:Journal Article
- Keywords:
Bacillus,Elastase,Screening,Identification
- From:
Microbiology
2008;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
The study provide a theoretical basis for the industrial production of a elastase-high-yield strain which was isolated from the straw,and the selected strain was identified. The screening strategy included casein(skim milk) plate selecting and elastin(beef tendon) re-screening. And then,the morphological,physiological and biochemical characteristics as well as 16S rRNA sequence homology of the selected strain were studied. Finally,the strain LSF-97 which had a excellent decomposing ability to beef tendon was obtained. The results showed that the strain LSF-97 is relative to the Bacillus pumilus with 100 % similar in sequence under the phylogenetic tree,the morphological and physiological and biochemical characteristics are also consistent with pattern of bacteria. So it was identified as Bacillus pumilus.
