PCR-SSCP Analysis of the Pronucleus Microbial Communities in Luzhou-flavor Daqu
- VernacularTitle:浓香型大曲原核微生物群落的PCR-SSCP解析
- Author:
Hui-Bo LUO
;
Zhi-Guo HUANG
;
Hao LI
;
Qiang ZHANG
;
Chun-Hui WEI
;
- Publication Type:Journal Article
- Keywords:
Daqu,PCR-SSCP,16S rDNA,Microflora
- From:
Microbiology
2008;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
PCR-SSCP(single-strand conformation polymorphism) was used for studying the community changes of pronucleus microorganisms in various fermenting stages of Luzhou-flavor Daqu. The results showed:(1) The pronucleus microorganism's community was similar and also had the polymorphism in each simple of various fermentation stage;(2) Different microflora had complex ecology effects of coordination and the restriction;(3) The diversity indexes of different stage of Daqu microorganisms were around 1.69～2.01,and the composition of them was stable relatively;(4) The similarity indexes were 0.67～1.00,and much higher in the approaching stages.
