Tandem Repeat Expression and Inhibitory Activity of a ?-lactamase Inhibitory Peptide
- VernacularTitle:?-内酰胺酶抑制多肽的串联表达及抑制活性研究
- Author:
Ming-Fei XU
;
Li-Ping XIE
;
Tao YANG
;
Chun-Bao ZHU
;
Bao-Quan ZHU
;
You-Jia HU
;
- Publication Type:Journal Article
- Keywords:
?-lactamase inhibitor Tandem repeat expression ?-lactamase
- From:
China Biotechnology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
?-lactamase inhibitor research is popular for its potential on ?-lactam antibiotics resistant strain.A ?-lactamase binding peptide SIPIS04-01 was obtained by the yeast two-hybid system.In vitro assay showed that it can inhibit the ?-lactamase activity.In order to improve the expression level of the recombinant peptide,a two-copy expression plasmid pYG563 was constructed by random orientation tandem repeat method after codon modification,the two-copy plasmid was successfully expressed and the product was increased by 48.4% than that of one-copy plasmid.Purified peptide showed inhibitory activity against TEM-1 ?-lactamase in vitro and the inhhibitory constant Ki was measured.
