Proteomics Analysis of Heteromorphic Leaves of Populus euphratica Oliv
- VernacularTitle:胡杨异形叶的蛋白质组学研究
- Author:
Ning YUE
;
Cai-Xia ZHENG
;
Xue BAI
;
Jian-Qing HAO
;
- Publication Type:Journal Article
- Keywords:
Populus euphratica Oliv Heteromorphic leaves Proteomics
- From:
China Biotechnology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
By exploring the different components of the lysis buffer and optimize the 2-DE conditions,established the best proteomics technical system for Populus euphratica's heteromorphic leaves,while take the heteromorphic leaves in the same blanche as the test materials to find differences between the protein expressions of the leaves.It showed that the lysis solution which containing 2mmol/L thiourea,7mmol/L urea,2% CHAPS,60mmol/L DTT and 0.2% IPG buffer could dissolve the protein better.Through tandem mass spectrum,the results show that heteromorphic leaves are different in photosynthesis and respiration.This research offered valuable informations for understanding the molecular mechanism during leaves development and elucidating the mechanism of the eco-adaptability of Populus euphratica.
