Mutational Screening of Zuelacmycin-producing Strain RL-2
- VernacularTitle:瑞拉菌素产生菌RL-2的诱变育种
- Author:
Li-Xia GOU
;
De-Rong AN
;
Shuang-Fa LIU
;
Jing LI
;
- Publication Type:Journal Article
- Keywords:
Streptomyces, Mutagenesis, Zuelacmycin, Antibaterial activity
- From:
Microbiology
2008;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
In this research, the zuelacmycin-producing strain Streptomyces venezuelaevar. qinlingensis RL-2 was used as original strain, the spore suspension of which was induced by UV, LiCl and the compound treatmeat (UV+LiCl) respectively. The zuelacmycin-high-yield strain UVL-108 was obtained by the treatment that the exposure time under UV irradition was 45 s and the concentration of LiCl was 0.4%. The heredity characters of mutant UVL-108 were stable in succesive six generations. The antibacterial activity and the fermentation titer of mutant UVL-108 were determinded by bidirectioned culture and mycelial linear growth respectively. The results demonstrated the antibaterial activity of mutant UVL-108 was improved by 77%, and the relative toxicity of fermentation to Phyricularia grisea was improved by two times compared with the original strain.
