Study on the Interaction of Gliotoxin with BSA
- VernacularTitle:胶毒素与BSA的相互作用
- Author:
Jun-Jie CHEN
;
Mei YANG
;
Lian-Ru ZHANG
;
Zhong-Hui ZHENG
;
Si-Yang SONG
;
- Publication Type:Journal Article
- Keywords:
Gliotoxin, Fluorescent spectrum, CD specrum, UV-Vis spectrum
- From:
Microbiology
2008;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
The interaction between Gliotoxin and bovine serum albumin (BSA) was studied by the fluo-rescence, Circular Dichroism (CD) and ultraviolet visible (UV-Vis) techniques. The fluorescent experiment showed that the intrinsic fluorescence of BSA was quenched by the binding of gliotoxin in a static quenching procedure, with an association constant of 7.2?103 L/mol and in hydropobic forces. And the CD spectrum revealed that gliotoxin effected the conformation of BSA by increased the mass of ?-helix.
