The Construction Strategy for Tandem Polypeptide Genes
- VernacularTitle:多肽串联基因构建策略
- Author:
Jun ZHANG
;
Ya-Lin YANG
;
Zi-Gang TIAN
;
Jian-Hua WANG
;
- Publication Type:Journal Article
- Keywords:
Polypeptide Multimers Multi-copy Tandem Construction
- From:
China Biotechnology
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
The tandem expression vector can effectively increase the expression level and structure stability of the target peptides(protein)with small size of molecular,and avoid toxicity towards host cell from some toxic peptides product,therefore,this approach is widely applied in biotechnology.The four construction methods of tandem expression plasmid including asymmetric and complementary cohesive ends,directional adapter,isocaudarners,and tandem expression cassette were reviewed in terms of protocol,characteristic and applicable field.In addition,selection principles from various construction methods of tandem plasmid were reviewed in terms of efficiency,accuracy and product cleavage.
