Study on the High Yield Strain of Gentamicin Producer by Protoplast Fused and Fermentor Test
- VernacularTitle:庆大霉素产生菌原生质体融合高产株与发酵罐试产的研究
- Author:
Yu-Xia GUAN
;
Ji-Xian LAN
;
Guang-Xin YAN
;
Jing-Shi ZHENG
;
- Publication Type:Journal Article
- Keywords:
Micromonospora purpurea Protoplast mutagenesis fusion Screened resistance Yield of fermentor
- From:
China Biotechnology
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
The protoplasts of Micromonospora purpurea,the high yield strains of gentammicin producer were mutagenized by diethyl sulfate(DES)and ultraviolet radiation(UV)respectively,then fused,screened by gentamicin resistance and regenerated.The average fermentation unit 2200?U/ml could be achieved by shake flask for 10 batches.The average fermentation unit 1900?U/ml could be obtained by 5L fermentor for 7 batches.The quality of the end product conformed to CP2000,BP2000 and USP26 pharmacopoeia.
