Deletion of dnd Gene Cluster in Salmonella enterica and Heterogenous Expression
- VernacularTitle:沙门氏菌中dnd基因簇的缺失与异源表达
- Author:
Tie-Gang XU
;
Fen YAO
;
Xiu-Fen ZHOU
;
De-Lin YOU
;
Zi-Xin DENG
;
- Publication Type:Journal Article
- Keywords:
Salmonella, DNA Phosphorothioation, DNA degradation, Gene deletion
- From:
Microbiology
2008;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Salmonella enterica serovar Cerro 87, which was isolated from a commercial egg-producing farm, has a phosphorothioated DNA backbone resulting DNA degradation(Dnd) during the pulsed field gel electrophoresis (PFGE) process. In this research, a gene deletion mutant XTG103 was engineered with the entire dnd gene cluster knocked out by double crossover using vector pKOV-kan, and lost Dnd phenotype corre- spondingly. We regulated the DNA phosphorothioation by heterogenous expression of dnd gene cluster with an isopropyl ?-D-1-thiogalactopyranoside (IPTG) inducible promoter PlacZ.
