Screening of ?-Rhamnosidase High-yield Strain from Aspergillus niger
- VernacularTitle:黑曲霉?-鼠李糖苷酶高产菌株的选育
- Author:
Hua-Gen CHEN
;
Hui NI
;
Li-Jun LI
;
An-Feng XIAO
;
Wen-Jin SU
;
Hui-Nong CAI
;
- Publication Type:Journal Article
- Keywords:
Aspergillus niger, ?-rhamnosidase, Mutation breeding, Transparent circle, Fermentation
- From:
Microbiology
2008;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
In this study, plate transparent circle by Davis method was introduce firstly screening ?-Rhamnosidase high-yield strain. The spore-sprouted Aspergillus niger 8-hour were mutagenized by ethyl methane sulphonate and pre-screened via transparent circle. 11% mutants yield 40% higher of ?-rhamnosidase than the original strain. A high-yield strain, T-226 with the highest ?-rhamnosidase activity of 373.4 U/mL was finally selected from these potential high-yield mutants after rescreened by shake flask fermentation twice. When the T-226 strain was fermented in 5 L bioreactor, the enzyme activity could reach to 631.9 U/mL after 84 h. Thus, the established screening method is highly efficient to isolate ?-rhamnosidase high-yield mutant of A. niger.
