Identificition of Endophytic Bacteria EBS05 from Cinamonum camphra and Property of Its Antimicrobial Compound
- VernacularTitle:樟树内生细菌EBS05的鉴定及其抗菌活性物质性质的研究
- Author:
Cai-Yi WEN
;
Zhi-Gang YIN
;
Jian-Guang CHEN
;
Hong-Lian LI
;
- Publication Type:Journal Article
- Keywords:
Plant endophytic bacteria, Bacillus subtilis, 16S~23S rRNA ISR, Antimicrobial activity
- From:
Microbiology
2008;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
An endophytic bacterium strain EBS05 from Cinamonum camphra was identified as Bacillus subtilis by morphological taxonomy and sequence analysis of 16S~23S rRNA intergenic spacer regions. Properties of antimicrobial compound produced by EBS05 were assayed. The active compound had the maximum absorbance peak at ?213.5 nm. The antimicrobial activity was stable in solution with pH value from 5 to 8, and decreased significantly in solution with pH value less than 4.0 or more than 9.0. The antimicrobial compound had thermodynamics stability. Its activity changed a little after treated at 60?C~80?C for two hours, and compared with 65% original activity after treated at 1?105 Pa for 30 minutes. The active substance had high resistance to ultraviolet radiation and protease K. Antimicrobial compound was soluble in alcohol solu- tion, which was easily dissolved in methanol and ethanol, but not dissolved in ethyl acetate, acetonitrile and petroleum et al.
